Abstract:Phenolic compounds, a highly toxic group of organic pollutants, pose significant risks to ecological security and human health. Their detection and elimination is crucial. Laccase, a polyphenol oxidase, efficiently catalyzes polyphenol oxidation to produce water, showing green and efficient biocatalytic properties valuable in biosensing and pollutant degradation. Practical use of laccase is hindered by high cost, poor stability, and recycling challenges. Nanozymes, functionally similar to natural enzymes, can address these drawbacks. Copper-based mimetic enzyme materials, inspired by laccase""s copper-containing active center, have emerged as effective laccase mimics. This article introduces laccase activity evaluation methods and discusses copper-based organic hybrid nanozymes with various ligands such as small molecules, nucleotides, and amino acids, exploring their impact on laccase activity. Progress in using copper-based organic hybrid materials for phenolic compound detection and degradation is summarized, along with forecasted trends for developing copper-based organic hybrids with laccase-like activity.